We narrowed to 2,845 results for: rela

1. pET30Xa-SBL1(160E,NoCys)

   Plasmid

   #66875

   Purpose

   Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   S160E; changed 4 (all) cysteines to serine

   Promoter

   T7lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PA-mCherry-miniSOG-H2B-C-10

   Plasmid

   #57789

   Purpose

   Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pSP64-hERG1a S620T

   Plasmid

   #53059

   Purpose

   Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Serine 620 to Threonine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX41

   Plasmid

   #46851

   Purpose

   TOP Hupki-wt plasmid without selection marker

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB and Hupki p53 wild type (TP53 Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)mGAT1CFP45

   Plasmid

   #41676

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” CFP A206K mutation; 45 C-terminal-…

   Promoter

   CMV

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)mGAT1YFP45

   Plasmid

   #41675

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; 45 C-terminal-…

   Promoter

   CMV

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1Xpress-AbrR683AN795A-HisC

   Plasmid

   #32510

   Depositor

   John Groffen, Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   ABR (ABR )
   

   Use

   Tags

   HISC

   Expression

   Mammalian

   Mutation

   R 683 to A and N 795 to A

   Promoter

   Available since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET28aAviHisCD72aCTLD

   Plasmid

   #129065

   Purpose

   generation of biotinylated mouse CD72a CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Article

   Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

   Insert

   CD72a (Cd72 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-2NLS-Cas9-6XMS2

    Plasmid

    #166033

    Purpose

    Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-SMS2-TEV-Twin-Strep

    Plasmid

    #202529

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS2 (SGMS2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TGA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiGuide-Hygro-eGFP

    Plasmid

    #99375

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHis-BRD4 BD1

    Plasmid

    #196544

    Purpose

    Expression of BRD4 Bromodomain (BD1) in E.coli

    Depositor

    Byung Il Lee

    Article

    Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

    Insert

    BRD4 BD1 (BRD4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRD405

    Plasmid

    #163110

    Purpose

    Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mScarlet-I_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMYH9-D1424H-FLAG

    Plasmid

    #183513

    Purpose

    Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Aspartic acid 1424 to Histidine

    Promoter

    CMV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

    Plasmid

    #193728

    Purpose

    Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

    Depositor

    Colin Akerman

    Article

    Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

    Insert

    ClopHensorN
    

    Use

    AAV

    Tags

    Strep-II (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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