168,037 results

1. pAAV.hSyn.iGluSnFR3.v857.PDGFR

   Plasmid

   #178329

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn iGluSnFR3 v857.PDGFR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and PDGFR TM Domain

   Expression

   Mammalian

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAdTrack-CMV

   Plasmid

   #16405

   Purpose

   Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV.TBG.PI.Cre.rBG

   Plasmid

   #107787

   Purpose

   AAV vector expressing Cre from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TBG

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFR_HCV_xb

   Plasmid

   #11510

   Depositor

   Phil Sharp

   Article

   Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

   Insert

   Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
   

   Use

   Luciferase

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-EGFP-puro

   Plasmid

   #45567

   Depositor

   Michael McVoy

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   IRES-EGFP-Puro

   Expression

   Mammalian

   Promoter

   Phcmv (strong human cytomegalovirus immediate ear…

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_BiPVe4_dTomato_nlsdTomato (AAV PHP.eB)

   Viral Prep

   #213936-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_dTomato_nlsdTomato (#213936). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Tags

   dTomato

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

8. TRUPATH Triple GaoB

   Plasmid

   #196052

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaoB-RLuc8
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gs(short)-CASE

   Plasmid

   #168124

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG1

   Expression

   Mammalian

   Mutation

   NLuc is inserted at N136/V137 within GNAS

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRB-mCherry-FTH1

    Plasmid

    #100749

    Purpose

    Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    ferritin heavy chain 1 (FTH1 Human)
    

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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