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pDONR223-GALK1

Plasmid

#23743

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GALK1 (GALK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K15

Plasmid

#23499

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K15 (MAP3K15 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCheY Tc delta C

Plasmid

#13520

Depositor

James McKerrow

Publication

Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

Insert

cruzain

Tags

CheY

Expression

Bacterial

Mutation

Truncated variant: stop codon after Gly212

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA

Plasmid

#235678

Purpose

Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

Depositor

Simon Powell

Publication

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Type

Empty backbone

Expression

Mammalian

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304-MARK3_T211A

Plasmid

#107236

Purpose

Lentiviral gene expression vector for phospho-MARK3_T221A expression

Depositor

Alex Kentsis

Publication

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Expression

Mammalian

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2193

Plasmid

#67542

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pKEB31

Plasmid

#31224

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

ccdB and Cm resistance gene

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Publication

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Expression

Insect

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

Plasmid

#136543

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Publication

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Publication

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN2

Plasmid

#125782

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Publication

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Tags

DD

Expression

Insect

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

Plasmid

#71812

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Publication

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC

Expression

Insect

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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BASIC_7_J23101-RBS34-mCherry-B0015

Plasmid

#68141

Purpose

storage vector for BASIC part: RFP expression cassette

Depositor

Geoffrey Baldwin

Publication

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS34-mcherry-B0015

Expression

Bacterial

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hIRmut-GFP

Plasmid

#22397

Depositor

Joseph Bass

Publication

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

IRmut

Tags

GFP

Expression

Mammalian

Mutation

changed Leu 62 to Proline

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-YSK4

Plasmid

#23616

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

YSK4 (YSK4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT11

Plasmid

#200991

Purpose

pLv1-pUC18R6KT-miniTn7-Gm, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKESaPar

Plasmid

#211829

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Publication

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTHSSe_53

Plasmid

#109251

Purpose

pUC BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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