We narrowed to 12,079 results for: ARIA;

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pCX-Lyn-SponGee

Plasmid

#134776

Purpose

mammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

Lyn-SponGee

Tags

FLAG and mRFP

Expression

Mammalian

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmAGO1

Plasmid

#22768

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcPylRS(Y384F Y306A)

Plasmid

#196486

Purpose

To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7_dead_mt

Plasmid

#167267

Purpose

Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Mutation

D106A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b2

Plasmid

#13752

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b2 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDL001_Hec1-ms_IgG_HC

Plasmid

#193618

Purpose

Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)

Tags

His6 and signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDL002_Hec1-ms_IgG_LC

Plasmid

#193619

Purpose

Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Hec1 light chain (Hec1-ms_IgG_LC)

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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645 pCS2-etv2

Plasmid

#49005

Purpose

Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Invitro transcription, riboprobe template

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP–COSMO–PB

Plasmid

#169684

Purpose

COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

Depositor

Yubin Zhou

Article

Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

Insert

anti-caffeine nanobody variant

Tags

modified STIM1–PB tag

Expression

Mammalian

Mutation

Y104W

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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K20‐scFv‐pSMBP2

Plasmid

#159940

Purpose

To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

Depositor

Sandra Schmid

Article

Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

Insert

anti beta-1 integrin scFv

Tags

6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-LbCas12a-Plus-mCherry

Plasmid

#191230

Purpose

To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

Depositor

Zhili Rong

Article

Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

Insert

LbCas12a-Plus-P2A-mCherry

Use

CRISPR

Mutation

D156R, R883K, R887A

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pOP459

Plasmid

#85933

Purpose

Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 2G4 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta

Plasmid

#64676

Purpose

expresses Wild type WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-TO-HA-SH3BP1 (RNAi-res)

Plasmid

#136645

Purpose

Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pOP462

Plasmid

#85935

Purpose

Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 13C6 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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DEP-GFP

Plasmid

#109043

Purpose

Expression of the Dishevelled DEP domain

Depositor

Mariann Bienz

Article

Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

Insert

dishevelled segment polarity protein 2 (DVL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Dvl2 DEP domain

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b1

Plasmid

#13756

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

Plasmid

#136646

Purpose

Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1R312A (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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