We narrowed to 80,162 results for: ELL

1. pSLQ1658-dCas9-EGFP

   Plasmid

   #51023

   Purpose

   Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   dCas9 fuse to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MSCV LTR promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Frankenbody-FLAG-Halo

   Plasmid

   #175296

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with HaloTag
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCiGSΔΨ

   Plasmid

   #108312

   Purpose

   Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

   Depositor

   David Russell

   Article

   Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

   Insert

   gag
   

   Expression

   Mammalian

   Mutation

   pCiGSΔΨ is a newer version of the pCiGS Gag expre…

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

   Plasmid

   #188384

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSin-EF2-Nanog-Pur

   Plasmid

   #16578

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens Nanog homeobox (NANOG Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.3 GCN1-3xFlag_IRES-iRFP

   Plasmid

   #198388

   Purpose

   GCN1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   GCN1 (GCN1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag‐mTim3

   Plasmid

   #49208

   Purpose

   expresses Flag tagged mouse Tim3

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (Havcr2 Mouse)
   

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   no Tim3 signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH1R-mCherry-N1

   Plasmid

   #84325

   Purpose

   Histamine-1 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H1R (HRH1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Integrin β3-GFP

   Plasmid

   #205091

   Purpose

   Express GFP-tagged human integrin β3 in mammalian cells

   Depositor

   Bianxiao Cui

   Article

   Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

   Insert

   Integrin beta 3 (ITGB3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PGK-HALO-CTERM-GFP

    Plasmid

    #185777

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGK-HALO-NTERM-GFP

    Plasmid

    #185772

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-Ccdc134-IRES-BFP

    Plasmid

    #117266

    Purpose

    Retroviral overexpression of Ccdc134

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Ccdc134 (Ccdc134 )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HAT1_AG

    Plasmid

    #217113

    Purpose

    Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HAT1_AG transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Plenti-ARE-HSVTK-luc

    Plasmid

    #161789

    Purpose

    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

    Depositor

    Hans-Guido Wendel

    Article

    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

    Inserts

    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EF1a and Murine Gclc ARE

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
    

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMaRSC

    Plasmid

    #89189

    Purpose

    The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    Flag-L274G-T2A-mCherry (Mars1 Mouse)
    

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-LAMP1

    Plasmid

    #207787

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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