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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53

Plasmid

#196267

Purpose

Mammalian expression of 3xHA tagged wild-type p53

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 (Trp53 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHCD1Dsol

Plasmid

#102955

Purpose

Mammalian expression of human soluble CD1D cDNA

Depositor

Gennaro De Libero, Lucia Mori

Article

Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

Insert

Human soluble CD1D cDNA (CD1D Human)

Tags

BirA

Expression

Mammalian

Mutation

Lacks the transmembrane region

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR223.1_mmDdx58 (closed)

Plasmid

#160107

Purpose

Expresses murine Ddx58 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ddx58 (Rigi Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_LacZ_sgRNA_2

Plasmid

#155093

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

Plasmid

#120561

Purpose

Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc Affimer6-msfGFPΔN12

Plasmid

#231550

Purpose

Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

msfGFP

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc Affimer6-sfCherry2ΔN12

Plasmid

#231551

Purpose

Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

sfCherry2

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti TMEM30A

Plasmid

#171789

Purpose

transfer plasmid for lentiviral vector production expressing TMEM30A

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6X His + Flag

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

Plasmid

#120562

Purpose

Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRECLV103_PGBD5_B

Plasmid

#168053

Purpose

Lentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G-trunc

Plasmid

#158077

Purpose

Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B

Plasmid

#203695

Purpose

expresses ATP10B

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAK-2

Plasmid

#210132

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAK-1

Plasmid

#210131

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614-trunc

Plasmid

#158076

Purpose

Expresses SARS-CoV-2 spike protein with a truncation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_LacZ

Plasmid

#155103

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCPF101-pfQC-WT

Plasmid

#184877

Purpose

expresses soluble plasmodium falciparum QC protein with a histag

Depositor

Ferenc Scheeren

Article

Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

pfQC-wt

Tags

His tag

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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