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Showing: 10441 - 10460 of 27539 results

  2. pGBW-m4046931

    Plasmid
    #145706
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145706-plasmid-map-sequence-id-307566

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  9. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70659-plasmid-map-sequence-id-162503

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  12. pET11-PC-H6

    Plasmid
    #183777
    Purpose
    Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.
    Depositor
    Peter Harte
    Article
    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113
    Insert
    Polycomb (Pc Fly)
    Tags
    6 His tag at the C-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    183777-plasmid-map-sequence-id-360072

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  13. pSilencer2.1-U6-3JB1R+6

    Plasmid
    #190842
    Purpose
    For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190842-plasmid-map-sequence-id-379236

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  14. pEPQD1CB0817

    Plasmid
    #177039
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
    Use
    Synthetic Biology
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177039-plasmid-map-sequence-id-349012

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  15. spa-GCaMP6f

    Plasmid
    #67558
    Purpose
    photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Tags
    HIS-tag
    Expression
    Mammalian
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    CMV
    Available Since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pMuLE ENTR U6-miR-30 R4-R3

    Plasmid
    #62117
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Expression
    Mammalian
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10441 - 10460 of 27539 results