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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,300 results for: crispr

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Showing: 10441 - 10460 of 25300 results

  1. snuABE4.2

    Plasmid
    #246014
    Purpose
    snuABE4.1 with MLH1-SB fused to the C-terminus via P2A.
    Depositor
    Sangsu Bae
    Publication
    Im et al bioRxiv 2025.07.19.664718
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Tags
    MLH1-SB
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246014-plasmid-map-sequence-id-492308

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  4. pQdCas9-sgempty

    Plasmid
    #159523
    Purpose
    Expression of dCas9 driven by lux cassette from Vibrio fischeri
    Depositor
    Radhakrishnan Mahadevan
    Publication
    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    159523-plasmid-map-sequence-id-315103

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  11. pOPO685

    Plasmid
    #195300
    Purpose
    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
    Depositor
    Joseph Peters
    Publication
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and native array with PaqCI entry site
    Expression
    Bacterial
    Promoter
    Arabinose inducible arapBAD
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195300-plasmid-map-sequence-id-394739

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  13. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid
    #136405
    Purpose
    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.
    Depositor
    Massimo Squatrito
    Publication
    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    BbsI restriction site at position 437 was removed…
    Promoter
    U6, PDK
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    136405-plasmid-map-sequence-id-269877

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  15. pQCascade-IS3

    Plasmid
    #140626
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Publication
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140626-plasmid-map-sequence-id-305994

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  18. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid
    #223123
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223123-plasmid-map-sequence-id-447704

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  19. pT3TS-Hf-RfxCas13d-HA

    Plasmid
    #234479
    Purpose
    Plasmid to carry out IVT of High-fidelity RfxCas13d (human codon-optimized)
    Depositor
    Miguel Angel Moreno-Mateos
    Publication
    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    HA
    Mutation
    Changed four alanine to valine in positions 134, …
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234479-plasmid-map-sequence-id-468663

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  20. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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Showing: 10441 - 10460 of 25300 results