We narrowed to 47,371 results for: cha;
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Plasmid#26049DepositorAvailable SinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only
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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Tags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAST-FRT-GFP-FRT-uMCas12a+nls1x
Plasmid#230908PurposeDouble inducible expression of Cas12a+ with Gal4 and Flp.DepositorInsertCas12a+
UseCRISPRExpressionInsectAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(mito-LD)
Plasmid#214421PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-3XHA-V5PromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161925PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyExpressionMammalianPromoterpEFAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable SinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a
Plasmid#53051PurposeExpresses alpha subunit of hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionPromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPS22B-Cl
Plasmid#160431PurposeExpresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoterDepositorInsertRPS22B (RPS22B Budding Yeast)
TagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to change nucleo…PromoterGAL1 and S. cerevisiae GAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_mScarlet
Plasmid#188039PurposeExpresses CapChR2-mScarlet in mammalian neuronsDepositorInsertCapChR2-mScarlet
UseAAVTagsmScarletMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianAvailable SinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-mCherry-enCas9(H840A)-PolI5M
Plasmid#249064PurposeExpresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertenCas9(H840A)-PolI5M
UseCRISPRExpressionMammalianAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-STChRger2-TS-EYFP
Plasmid#129395PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianPromoterhSynAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_KanR neo
Plasmid#167877PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
TagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A
Plasmid#110555PurposepFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.DepositorInsertAnti-RD114 heavy chain
TagsmIgG2AExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERalpha-5Z
Plasmid#23061DepositorInsertERalpha (ESR1 Human)
ExpressionYeastAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYP-GFP
Plasmid#197283PurposeEncodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.DepositorInsertSYP and GFP (Syp Rat)
UseLentiviralTags1D4 epitopeMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
ExpressionMammalianMutationA215CPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only