We narrowed to 18,382 results for: URE

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RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

Plasmid

#139445

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-C

Plasmid

#30224

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with C terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn-HpaBC

Plasmid

#100949

Purpose

Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Inserts

RgTALsyn
HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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Krox20-MSE8 pGL3-TATA

Plasmid

#21261

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

Krox20-Myelin Specific Enhancer (Egr2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
TNF-SBP-mCherry_puromycin

Plasmid

#65285

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean-LaminB1-10

Plasmid

#55380

Purpose

Localization: Nuclear Envelope, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LaminB1 (LMNB1 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BATF3

Plasmid

#67050

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BATF3 (BATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CBP TAZ1(340-439)

Plasmid

#173760

Purpose

bacterial expression of mouse CBP TAZ1 domain

Depositor

Peter Wright

Article

De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

Insert

CBP TAZ1 (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0374 (His-MBP-Cas8_Cas7_Cas6)

Plasmid

#130639

Purpose

Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SKAP2-1/1

Plasmid

#91447

Purpose

Protein expression and purification of human SH3 domain construct SKAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SKAP2-1/1 (SKAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pETM33_MAD2L1_HORMA

Plasmid

#178480

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

MAD2L1_HORMA (MAD2L1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHCMV_VSVgSS-diCR2

Plasmid

#207324

Purpose

Expression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infection

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

diCR2 (LDLR Human)

Expression

Mammalian

Mutation

fragment of LDL-R dimerized

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-mCherry-attP(C31)-*BleoR

Plasmid

#183752

Purpose

phiC31 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

FRT-mCherry-attP-BleoR-F3

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

Plasmid

#187358

Purpose

Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

HPC-4 antibody heavy chain, and 6xHIS tag

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_TNKS_TANK-peptide-bind

Plasmid

#178489

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

TNKS_TANK-peptide-bind (TNKS Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCINeoFlag ALIX; I318A

Plasmid

#89867

Purpose

Expresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

ALIX (PDCD6IP Human)

Tags

Flag

Expression

Mammalian

Mutation

Mutated isoleucine 318 to alanine

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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