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HT028_FCMV_CheRiff_EGFP

Plasmid

#208605

Purpose

Expressing CheRiff_EGFP under CMV promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRiff_EGFP

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCgVchCAST-crtYf

Plasmid

#203816

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PhoDer

Plasmid

#188473

Purpose

Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

Depositor

Gary Mo

Article

Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

Insert

PhoDer (GSDMD Human, Synthetic)

Expression

Mammalian

Promoter

CMV IE

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-BirA-HA

Plasmid

#232587

Purpose

Express BirA tagged to the C-terminus of CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 FL

Plasmid

#17648

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Plasmid

#238244

Purpose

For integration of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pEGFP-C1

Plasmid

#71378

Purpose

For analysis of PH domain localization in mammalian cells

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin homology (PH) domain; aa 247-399

Promoter

CMV

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSV human p85 alpha (HA tag)

Plasmid

#11499

Depositor

Ronald Kahn

Article

Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

Insert

p85 alpha (PIK3R1 Human)

Tags

HA

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoG

Plasmid

#238235

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Article

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Article

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pK.myc-MYPT1

Plasmid

#24101

Depositor

Masumi Eto

Article

Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

Insert

Myosin-targeting subunit-1 (PPP1R12A Human)

Tags

Myc

Expression

Mammalian

Mutation

S50G, P415Q

Available Since

March 19, 2010

Availability

Academic Institutions and Nonprofits only

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pDriver

Plasmid

#165149

Purpose

Driver plasmid for PROBER encoding SV40 Large T-antigen

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

SV40 Large T-antigen

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Article

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Tags

Strep(II)-tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pISCba-reRNA

Plasmid

#226823

Purpose

Expressing ISCba controled by PlacM promoter, guide RNA controled byPJ23119 promoter

Depositor

Sheng Yang

Article

Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

Insert

ISCba and its corresponding guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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