We narrowed to 13,693 results for: crispr cas9

1. pORANGE GFP-Clta KI

   Plasmid

   #131483

   Purpose

   Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Clta Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_antiCD19_eZ3_Gal4VP64

   Plasmid

   #169917

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Additional EGF repeat between the extracellular d…

   Promoter

   PGK

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

4. BlastR-P2A-DD-TCOF1-donor

   Plasmid

   #60748

   Purpose

   Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. HR-TERT-SV40-GFP

   Plasmid

   #71397

   Purpose

   Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Tags

   SV-40 GFP insertion in TERT promotor

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-DMD-Donor

   Plasmid

   #60605

   Purpose

   Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   Hygromycin resistant gene
   

   Use

   Knock-in donor vector

   Expression

   Mammalian

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE Shank1-GFP KI

   Plasmid

   #131500

   Purpose

   Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Shank1, Shank1 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

   Plasmid

   #160624

   Purpose

   TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2:VPR:Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE Rims2-GFP KI

   Plasmid

   #131495

   Purpose

   Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Rims2, Rims2 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE Shank2-GFP KI

    Plasmid

    #131496

    Purpose

    Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank2, Shank2 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. px330 Rosa26 Gu1 (LH416)

    Plasmid

    #99629

    Purpose

    Plasmid used to target the wildtype Rosa26 allele with Cas9

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE Doc2A-GFP KI

    Plasmid

    #131478

    Purpose

    Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Doc2a Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3s-ABE7.10

    Plasmid

    #113128

    Purpose

    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE 7.10
    

    Tags

    HA epitope and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. human ETV6 gRNA-1

    Plasmid

    #133353

    Purpose

    human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gfap-EGFP donor

    Plasmid

    #65564

    Purpose

    Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    gfap-EGFP (gfap Zebrafish)
    

    Use

    Zebrafish injection

    Promoter

    none

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORANGE Cplx2-GFP KI

    Plasmid

    #131476

    Purpose

    Endogenous tagging of Complexin2: C-terminal (amino acid position: L128)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cplx2 Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid

    #75411

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [M1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

    Plasmid

    #160622

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s_MS2:EDLL_Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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