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Syntaxin1A(E49TAG,G42H,K46H)-mCherry

Plasmid

#241292

Purpose

Mammalian expression of syntaxin-1(E49TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG,G42H,K46H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E53TAG,G42H,K46H)-mCherry

Plasmid

#241294

Purpose

Mammalian expression of syntaxin-1(E53TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG,G42H,K46H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(R210H,E206TAG)-mCherry

Plasmid

#241296

Purpose

Mammalian expression of syntaxin-1(E206TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E206TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(R210H,K84TAG)-mCherry

Plasmid

#241298

Purpose

Mammalian expression of syntaxin-1(K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K84TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(I233A,K84TAG)-mCherry

Plasmid

#241299

Purpose

Mammalian expression of syntaxin-1(I233A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

I233A,K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(I233A,R210H,K84TAG)-mCherry

Plasmid

#241300

Purpose

Mammalian expression of syntaxin-1(I233A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

I233A,K84TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K264A,K265A,K84TAG)-mCherry

Plasmid

#241301

Purpose

Mammalian expression of syntaxin-1(K264A,K265A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K264A,K265A,K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pNB-Rep

Plasmid

#238996

Purpose

Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-pm-mKate2

Plasmid

#245521

Purpose

Plasmid for PiggyBac /ITR mediated insertion of pm-mKate2 sequence downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

pm-mKate2

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#244921

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-F-Tractin-mKate2

Plasmid

#245523

Purpose

Plasmid for PiggyBac /ITR mediated insertion of F-Tractin sequence downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

F-Tractin-mKate2

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-pm-eGFP

Plasmid

#245520

Purpose

Plasmid for PiggyBac /ITR mediated insertion of pm-eGFP downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

pm-eGFP

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mStayGold

Plasmid

#234549

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mStayGold(Ando) fusion protein

Depositor

Hajime Hirase

Article

Knak et al Neurophotonics, Vol. 12, Issue S2, S2281

Insert

Alb-mStayGold

Use

AAV

Mutation

None

Promoter

P3

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215329

Purpose

Lentiviral mammalian vector with firefly luciferase gene with no upstream promoter sequence; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

No Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mRuby3

Plasmid

#244911

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mRuby3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mIRFP670nano3

Plasmid

#244917

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mIRFP670nano3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mIRFP670nano3

Plasmid

#244916

Purpose

iATPSnFR2.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mIRFP670nano3

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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Stx3-mCherry

Plasmid

#190890

Purpose

Expresses syntaxin 3 fused to mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mRuby3

Plasmid

#244912

Purpose

iATPSnFR2.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mRuby3

Use

AAV

Mutation

A95K

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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