Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,991 results for: LEA

Showing: 2601 - 2650 of 15991 results

  3. pcDNA3-Mcm5-mCer

    Plasmid
    #211393
    Purpose
    mammalian expression of human Mcm5 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm5 (MCM5 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211393-plasmid-map-sequence-id-420429

    Enlarge

  11. NFATC2IP

    Plasmid
    #28284
    Depositor
    Cheryl Arrowsmith
    Insert
    NFATC2IP (NFATC2IP Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains N-terminal ubiquitin-like domain (residu…
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. GS1.2/ADPGPP-350

    Plasmid
    #140418
    Purpose
    mApple expression regulated by the A. thaliana UBQ10 promoter, mCitrine expression regulated by a truncated A. thaliana ADPGPP promoter. nourseothricin acetyl transferase selectable marker (nat)
    Depositor
    Mathias Pribil
    Inserts
    mApple
    mCitrine
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    A. thaliana UBQ10 and A. thaliana truncated ADPGPP
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    140418-plasmid-map-sequence-id-297076

    Enlarge

  13. pcDNA3.1-hSyncytin-1(AAAR)

    Plasmid
    #182442
    Purpose
    hSyncytin-1, furin cleavage site RNKR>AAAR(314-317)
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Expression
    Mammalian
    Mutation
    furin cleacage site RNKR>>AAAR(314-317)
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182442-plasmid-map-sequence-id-357724

    Enlarge

  14. pT7CFE1_EVD68_VP12A_C107R

    Plasmid
    #212982
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A C107R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed cysteine 107 from 2A to Arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212982-plasmid-map-sequence-id-422577

    Enlarge

  15. pcDNA3-Cdc45-mCer

    Plasmid
    #211389
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211389-plasmid-map-sequence-id-420426

    Enlarge

  16. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

    Enlarge

  17. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid
    #212980
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed cysteine 147 from 3C to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212980-plasmid-map-sequence-id-420353

    Enlarge

  18. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

    Enlarge

  19. pcDNA3-C17orf53-mCer

    Plasmid
    #211420
    Purpose
    mammalian expression of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211420-plasmid-map-sequence-id-419429

    Enlarge

  20. TALEN_C18

    Plasmid
    #47974
    Purpose
    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    Shortened C-Terminus,Codon Optimized FokI Endonuc…
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47974-plasmid-map-sequence-id-68905

    Enlarge

  23. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

    Enlarge

  24. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-130147

    Enlarge

  25. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

    Enlarge

  29. pcDNA3.1-hNurr1-NE

    Plasmid
    #102363
    Purpose
    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells
    Depositor
    Shu Leong Ho
    Insert
    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    Tags
    NE
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102363-plasmid-map-sequence-id-200857

    Enlarge

  35. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

    Enlarge

  36. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

    Enlarge

  42. pAAP2

    Plasmid
    #171208
    Purpose
    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    pgl-3 (pgl-3 Nematode)
    Tags
    MBP::HIS::TEV cleavage site
    Expression
    Bacterial
    Available Since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171208-plasmid-map-sequence-id-335799

    Enlarge

  46. pG1AK

    Plasmid
    #71736
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71736-plasmid-map-sequence-id-130104

    Enlarge

Showing: 2601 - 2650 of 15991 results