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Addgene

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We narrowed to 39,438 results for: Eras

Showing: 1041 - 1060 of 39438 results

  4. Syn61

    Bacterial Strain
    #174513
    Purpose
    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1
    Depositor
    Jason W Chin
    Article
    Fredens et al Nature. 2019 May;569(7757):514-518. doi:
    Bacterial Resistance
    Streptomycin
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  5. pESR1-donor

    Plasmid
    #112337
    Purpose
    CRISPR donor plasmid to tag human transcription factor ESR1 with GFP
    Depositor
    Kevin White
    Insert
    ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    112337-plasmid-map-sequence-id-241244

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  6. pESR1.1.0-gDNA

    Plasmid
    #112433
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1
    Depositor
    Kevin White
    Insert
    ESR1 (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112433-plasmid-map-sequence-id-230374

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  10. BM28

    Bacterial Strain
    #201047
    Purpose
    MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.
    Depositor
    Jeannette Winter
    Article
    McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi
    Bacterial Resistance
    Ampicillin and kanamycin
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Use
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  13. KJ740(DE3)

    Bacterial Strain
    #179486
    Purpose
    KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Bacterial Resistance
    Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)
    Species
    Escherichia coli
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. RF21

    Bacterial Strain
    #102803
    Purpose
    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)
    Depositor
    Toshio Iwasaki, Robert Gennis
    Article
    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d
    Bacterial Resistance
    None
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units
    Depositor
    Diego Orzaez
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Ian Frew
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    A set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.
    Depositor
    David Sabatini, Kris Wood
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1041 - 1060 of 39438 results