We narrowed to 18,382 results for: URE

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pJ207 Delta-cpc-KanR

Plasmid

#51468

Purpose

Deletes the cpc operon in Synechocystis and confers kanamycin resistance

Depositor

Anastasios Melis

Article

Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

Insert

Replacing the cpc operon with kanamycin resistance

Use

Synthetic Biology

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PSTPIP1-1/1

Plasmid

#91453

Purpose

Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PSTPIP1-1/1 (PSTPIP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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R9-WT

Plasmid

#125210

Purpose

Expressing wildtype human Rad9b gene

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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Psicheck2 MERTK full length 3'UTR

Plasmid

#26992

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

MERTK Full length 3' UTR (MERTK Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
RBM6_RRM_pGEX

Plasmid

#135157

Purpose

Recombinant expression of RBM6 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM6 (RBM6 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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mKO-Fibrillarin-7

Plasmid

#57842

Purpose

Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Fibrillarin (FBL Human)

Tags

mKO

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_PIK3R1-1/1

Plasmid

#91274

Purpose

Protein expression and purification of human SH3 domain construct PIK3R1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R1-1/1 (PIK3R1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
DNMT1 KO Hyg

Plasmid

#16648

Depositor

Bert Vogelstein

Article

Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

Insert

DNMT1 knock-out construct (DNMT1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
PMYT1_HUMAN_D0

Plasmid

#79687

Purpose

This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PMYT1

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Arp3_pLib

Plasmid

#173677

Purpose

Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp3 (ACTR3 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-F146A

Plasmid

#115616

Purpose

Expresses human Parkin F146A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

F146A

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHM033Env

Plasmid

#137672

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NEDD9-1/1

Plasmid

#91498

Purpose

Protein expression and purification of human SH3 domain construct NEDD9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEDD9-1/1 (NEDD9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pECE-HA-FOXO3 S574A

Plasmid

#52426

Purpose

mammalian expression of FOX03 with mutant JNK phosphorylation site

Depositor

Steven Weinman

Article

Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

S574A, mutant JNK phosphorylation site

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (925-953)

Plasmid

#73744

Purpose

Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock V315R

Plasmid

#47342

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

V315R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only