We narrowed to 30,073 results for: des.2

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pTrcHisA-rat-PTP1B

Plasmid

#35987

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

His and Xpress Epitope Tag

Expression

Bacterial

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template3-3XMyc-Spacer

Plasmid

#176210

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template3-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template2-3XMyc-Spacer

Plasmid

#176209

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template2-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSG7-H588A-NmeCas9

Plasmid

#71477

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-H588A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

H588A

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV HDM2(NES-)

Plasmid

#12087

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Nuclear-export sequence mutant (NES-): L205A, I20…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG425 GAL Pong TPase L418A, L420A

Plasmid

#145795

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock, Susan Wessler

Article

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

Pong TPase L418A, L420A

Expression

Yeast

Mutation

L418A, L420A

Available Since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBluescript goosecoid

Plasmid

#16935

Depositor

John Postlethwait

Article

Thisse et al Dev Biol. 1994 Aug . 164(2):420-9.

Insert

goosecoid (gsc Zebrafish)

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
mCas13 Twin-SUMO

Plasmid

#199753

Purpose

Expresses codon optimized miniature mCas13 for protein expression in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

Insert

mCas13

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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GST-RP2

Plasmid

#86072

Purpose

A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFTK087

Plasmid

#171359

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTK38_mCherry-LGN-C

Plasmid

#46346

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

LGN C-terminal region (GPSM2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

contains aa365-684; deletion of S524

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111926

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-Foxd3 in situ

Plasmid

#37098

Depositor

Patricia Labosky

Article

Labosky et al Mech Dev. 1998 Aug;76(1-2):185-90.

Insert

Foxd3 (Foxd3 Mouse)

Use

In situ hybridization probe

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
OeNL(Ca2+)_18μ-pcDNA3

Plasmid

#111928

Purpose

Orange color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

OeNL(Ca2+)_18μ

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111927

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK093

Plasmid

#171365

Purpose

Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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