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pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Expression

Mammalian

Mutation

E17K

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR18Q

Plasmid

#87228

Purpose

HTT HR arms with 18 CAG repeats and piggyBac selection cassette

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm

Expression

Mammalian

Promoter

EF1a driving selection cassette

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB2 200bmut

Plasmid

#35538

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB2 gene (ZEB2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

6 Mutations in the miR-200b seed regions of the Z…

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBa-flag-BicD2 594-FKBP

Plasmid

#64206

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-short_BrokenHeart (no BC)

Plasmid

#86950

Purpose

AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

Depositor

Joseph Dougherty, Rob Mitra

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

Brokenheart construct

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.H66A.L276V

Plasmid

#238944

Purpose

Green glucose sensor, high sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.L276V.K312A

Plasmid

#238945

Purpose

Green glucose sensor, moderate sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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TadA-NG-CBE

Plasmid

#227420

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

TadA-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac-FLAG-mProser1-IRES-Blast

Plasmid

#226172

Purpose

Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Tags

2X FLAG

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pICOZ-SB100X[EF1α-CD19 4-1BB]

Plasmid

#218328

Purpose

Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP wildtype

Plasmid

#73048

Purpose

C-terminal fusion of gfp Mouse wildtype Foxg1

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

mFoxg1 (Foxg1 Mouse)

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2DA

Plasmid

#12286

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2DA (PTPN11 Human)

Tags

Myc

Expression

Mammalian

Mutation

Aspartic Acid 425 mutated to Alanine

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE-FH-TET3 short

Plasmid

#166916

Purpose

Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

TET3 short isoform

Use

Lentiviral

Tags

HA Tag

Expression

Mammalian

Mutation

Changed nucleotide 18 (G) to (A) to delete FseI r…

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 (delta110)

Plasmid

#71861

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, missing first 110 amino acids

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pBa-eGFP-flag-BicD2 594-FKBP

Plasmid

#63569

Purpose

Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP, eGFP, and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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