165,817 results

1. pMA4008

   Plasmid

   #70109

   Purpose

   mtDNA destruction/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   Herpes simplex virus UL12.5M185
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   N-terminal deletion of UL12.5

   Promoter

   CMV and RSV

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-Ubiquitin-K29

   Plasmid

   #22903

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K29 only, all other lysines mutated to arginines

   Promoter

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 TEV LIC cloning vector (2B-T)

   Plasmid

   #29666

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. MoClo Plant Parts II and Infrastructure Kit

   Plasmid Kit

   #1000000135

   Purpose

   Compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.

   Depositor

   Johannes Stuttmann

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

5. paavCAG-post-mGRASP-2A-dTomato

   Plasmid

   #34912

   Purpose

   post

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Has Service

   AAV5

   Insert

   postsynaptic mGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-Solo-mStayGold-TOMM20

   Plasmid

   #227307

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV-SS-antiFLAGscFv-TM

   Plasmid

   #198303

   Purpose

   Displays anti-FLAG scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiFLAGscFv-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVDL9.3

   Plasmid

   #168299

   Purpose

   Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

   Depositor

   Víctor de Lorenzo

   Article

   Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

   Insert

   Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-LC3

   Plasmid

   #11546

   Depositor

   Karla Kirkegaard

   Article

   Jackson et al PLoS Biol. 2005 May . 3(5):e156.

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY100-RetroEFS-HER2-28BBz-WPRE

    Plasmid

    #192201

    Purpose

    Retro-HER2CAR

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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