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Plasmid#69115PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of 678-681aa region. Silent mutations i…PromoterCMVAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only
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pCSCX
Plasmid#63918PurposeExpression of the sucrose utilization phenotypeDepositorInsertcscAKB
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human Transporter Knockout Library with UMIs
Pooled Library#221409PurposegRNA library to test the impact of transporters on biological processesDepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
S. rosetta Col+ cDNA amplified library
Pooled Library#62844Purposeamplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensisDepositorExpressionBacterialSpeciesSalpingoeca rosettaAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
DmTmem63
Plasmid#136598PurposeExpresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cellsDepositorInsertDmTMEM63
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) eGFP
Plasmid#107505PurposeExpresses eGFP, puromycin resistantDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKAsub-TagRFPT-CAAX
Plasmid#87712PurposeEncodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorInsertPKAsub-TagRFPT-CAAX
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only