Addgene

We narrowed to 9,769 results for: otos

Showing: 1591 - 1620 of 9769 results

Results per page:

Sort results by:

pCAGGS-SspB-mScarlet-I

Plasmid

#178521

Purpose

Expresses SspB-mScarlet-I in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

SspB-mScarlet-I

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCruzHA SIRT1

Plasmid

#10962

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pZmUBQ:LUC

Plasmid

#131860

Purpose

Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

luciferase

Use

Tags

Expression

Plant

Mutation

Promoter

ZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-) FLIPQTV3.0 100micro

Plasmid

#63730

Purpose

FLIPQTV3.0 glutamine sensor with D157N mutation

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 100microM affinity

Use

Tags

Expression

Mammalian

Mutation

D157N in glutamine binding domain

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII

Plasmid

#165438

Purpose

Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Cre

Plasmid

#125574

Purpose

Expresses Cre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Marvin Bentley, Gary Banker

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-LOV*-MVP

Plasmid

#202206

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Major vault protein (MVP Human)

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP-AAV-ChATen-mCherry

Plasmid

#193741

Purpose

This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

Depositor

Hynek Wichterle

Insert

ChAT enhancer, mini promoter, synthetic intron, mCherry

Use

AAV and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

1000 bp ChAT enhancer with a 32 bp minimal promot…

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-LOV*-H2B

Plasmid

#202204

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Histone H2B type 1-K

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN3P_T3_ABEmax_IVT

Plasmid

#171761

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

ABEmax

Use

Vector for in-vitro transcription

Tags

Expression

Mutation

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE4

Use

Vector for in-vitro transcription

Tags

Expression

Mutation

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZmUBQ:GW

Plasmid

#132358

Purpose

Expression of CDS from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Type

Empty backbone

Use

Tags

YFP

Expression

Plant

Mutation

Promoter

pZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-HA-ERK5-FL

Plasmid

#31817

Depositor

Astar Winoto

Article

Kasler et al Mol Cell Biol. 2000 Nov;20(22):8382-9.

Insert

Erk5 (Mapk7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
GG-EBNA-OCT4

Plasmid

#69537

Purpose

Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

5 concatenated gRNA transcriptional cassettes targeting OCT4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Use

Tags

Lamp1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

NLS (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
MBP-CRY1-PHR

Plasmid

#26035

Depositor

Johann Deisenhofer

Article

Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

CRY1 (CRY1 Mustard Weed)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pTomo-AurA

Plasmid

#26293

Depositor

Inder Verma

Article

Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Insert

aurora kinase A (AURKA Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRTP801/TA-ODD

Plasmid

#206212

Purpose

An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

Depositor

Masamichi Kamihira

Article

Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

Insert

tetracycline-responsive transactivator fused with oxygen-dependent degradation domain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAiRFP2-N1

Plasmid

#44273

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP2

Use

Tags

Expression

Mammalian

Mutation

K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_D10A-1x7

Plasmid

#58777

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge