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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2651 - 2700 of 11682 results

  2. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  12. pACYCduet-mxLOX1-EH

    Plasmid
    #104978
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Inserts
    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Expression
    Bacterial
    Promoter
    T7promoter
    Available Since
    Feb. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    104978-plasmid-map-sequence-id-203225

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  17. pBP-Pveg

    Plasmid
    #112776
    Purpose
    Strong promoter active during sporulation and vegetative growth
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg promoter
    Expression
    Bacterial
    Available Since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112776-plasmid-map-sequence-id-218356

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  20. pEBG-II-I Y248,249,611F

    Plasmid
    #22251
    Depositor
    Ananda Roy
    Insert
    TFII-I (GTF2I Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Tyrosine 248, 249 and 611 mutated to Phenylalanine
    Available Since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  22. pBW003

    Plasmid
    #131804
    Purpose
    Vector with aTc inducible M. laminosus Fd1 flanked by BbsI restriction sites
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    Mastigocladus laminosus ferredoxin
    Use
    Synthetic Biology
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131804-plasmid-map-sequence-id-258986

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  23. pBP-Pars

    Plasmid
    #112779
    Purpose
    Promoter of ars operon. Repressed by ArsR, induced by arsenate or arsenite
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pars promoter
    Expression
    Bacterial
    Available Since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112779-plasmid-map-sequence-id-218364

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  24. pBCSK_LEU2_I

    Plasmid
    #109060
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    LEU2
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109060-plasmid-map-sequence-id-209546

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  36. pTet_PflHamMA(S157A)(deltaA)B

    Plasmid
    #248939
    Purpose
    Hachiman from P. fluorescens under pTet control with the dHamA domain removed and with a protease S157A mutation
    Depositor
    Jennifer Doudna
    Insert
    PflHamMA(S157A)(deltaA)B
    Expression
    Bacterial
    Mutation
    S157A, HamA domain deletion
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248939-plasmid-map-sequence-id-500773

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  37. pSBinit-Syb-BC10(Y107A/W108S)

    Plasmid
    #250412
    Purpose
    Expression of Anti-SLC66A2 Sybody Non-Binding Variant in Bacteria
    Depositor
    Simon Newstead
    Insert
    Syb-BC10
    Use
    Affinity Reagent/ Antibody
    Tags
    Myc-6xHis and PelB
    Expression
    Bacterial
    Mutation
    CDR3(Y107A/W108S)
    Promoter
    araBAD
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250412-plasmid-map-sequence-id-499039

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  38. pDest_PANCEd_AraC_R2_S170_LOV_P1.1_C3

    Plasmid
    #242154
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P1.1.3 variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV P1.1.3
    Expression
    Bacterial
    Mutation
    AraC: E3K, R146L, A152T, R251L, E281K; AsLOV2: D5…
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242154-plasmid-map-sequence-id-492357

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  41. DEXsynth:ccdb+ RUBY

    Plasmid
    #233320
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb+ RUBY
    Use
    Gateway destination
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233320-plasmid-map-sequence-id-464379

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  42. DEXsynth:ccdb+ mcherry

    Plasmid
    #233315
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb+ mcherry
    Use
    Gateway destination
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233315-plasmid-map-sequence-id-464362

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  43. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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  47. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  48. pJBL7345

    Plasmid
    #217372
    Purpose
    sfGFP reporter for chimeric riboswitch with Cbe pfl ZTP riboswitch aptamer and WT Pca rhtB ZTP riboswitch expression platform
    Depositor
    Julius Lucks
    Article
    Bushhouse et al bioRxiv 2024.03.29.587317
    Insert
    Chimeric ZTP riboswitch with Cbe pfl aptamer and WT Pca rhtB expression platform sfGFP reporter
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217372-plasmid-map-sequence-id-452545

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  49. GABAAR alpha5 HA/pCI

    Plasmid
    #197322
    Purpose
    Plasmid expressing an antigen targeting the GABA-A receptor alpha5 subunit
    Depositor
    Stephen Moss
    Insert
    GABA A alpha 5 (GABRA5 Human)
    Expression
    Mammalian
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197322-plasmid-map-sequence-id-392915

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Showing: 2651 - 2700 of 11682 results