Addgene

We narrowed to 10,181 results for: AGA

Showing: 1061 - 1080 of 10181 results

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Kohinoor-Golgi/pcDNA3

Plasmid

#67779

Purpose

Golgi-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

GT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Zyxin/pcDNA3

Plasmid

#67777

Purpose

Zyxin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Zyxin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M142A-invitro(E13)

Plasmid

#52017

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pLVTH-shNP1

Plasmid

#65062

Purpose

Knockdown of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

shNP1 (Nptx1 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_SAP130_1

Plasmid

#86310

Purpose

Encodes gRNA for 3' target of human SAP130

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SAP130 (SAP130 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCZ45ai

Plasmid

#16469

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

p53 binding site (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtAwoH

Plasmid

#65017

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2-U6.3-sgRNA-MFN1-GFP

Plasmid

#221846

Purpose

Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote

Depositor

Stephen Terry

Article

O’Sullivan et al bioRxiv 2024.03.04.583298

Inserts

EGFP
MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT

Use

CRISPR; Tol2 transposon optimised for chick expre…

Tags

Expression

Mammalian

Mutation

Promoter

CAGC and U6.3 chick

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-AC17-5c-frGFP

Plasmid

#217525

Purpose

AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

AC17-5c riboswitch-regulated folding reporter GFP

Use

In vitro transcription-translation

Tags

Expression

Mutation

Promoter

T7

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-Theo-3c-EGFP

Plasmid

#217526

Purpose

Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

Theo-3c riboswitch-regulated EGFP

Use

In vitro transcription-translation

Tags

Expression

Mutation

Promoter

T7

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_W267N

Plasmid

#214314

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

W267N

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_R269D

Plasmid

#214313

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

R269D

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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