We narrowed to 7,469 results for: Ank;

1. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a-SUMO2

   Plasmid

   #53142

   Purpose

   E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   SUMO2 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-92)

   Promoter

   T7

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0041-RanCas13b crRNA backbone

   Plasmid

   #103852

   Purpose

   For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-RanCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBDEST.IP

   Plasmid

   #79472

   Purpose

   Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Type

   Empty backbone

   Use

   Promoterless gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Lifeact EGFP

   Plasmid

   #187686

   Purpose

   In vivo visualization of actin

   Depositor

   Mary Beckerle

   Article

   Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. rTetR-GFP

   Plasmid

   #183916

   Purpose

   Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   rTetR-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Use

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDA019

   Plasmid

   #131452

   Purpose

   control expression vector

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH-tdGFP

   Plasmid

   #74322

   Purpose

   pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

   Depositor

   Joerg Stelling

   Article

   Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

   Insert

   pH-tdGFP
   

   Use

   Yeast tagging

   Tags

   none

   Expression

   Yeast

   Mutation

   N149Y, Q204H

   Promoter

   Available since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-gfap

    Plasmid

    #82401

    Purpose

    Gateway-compatible zebrafish gfap promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    gfap promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTetO-ISce1-TetO

    Plasmid

    #58506

    Purpose

    ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Tet repeats flanking ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK3_036

    Plasmid

    #123750

    Purpose

    Encodes mScarlet as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK2_005

    Plasmid

    #123700

    Purpose

    Encodes pCAG as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-BrainbowTEC

    Plasmid

    #82405

    Purpose

    Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Inserts

    tdTomatoMYC fusion
    EGFP
    E2CrimsonHA
    

    Use

    Tags

    HA and MYC

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK4_003

    Plasmid

    #123812

    Purpose

    Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    rglb pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-GFP-P2A

    Plasmid

    #80807

    Purpose

    GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP-P2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0090

    Plasmid

    #181954

    Purpose

    Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xTOPFlash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL059

    Plasmid

    #177210

    Purpose

    Bicistronic vector with GFP reporter

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJET1.2-STOP-dsRed

    Plasmid

    #60944

    Purpose

    Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    dsRED
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFW11-ffluc

    Plasmid

    #128487

    Purpose

    Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    firefly luciferase gene
    

    Use

    Allelic replacement vector

    Tags

    spy0535 downstream flanking region and spy0535 up…

    Expression

    Mutation

    Promoter

    synthetic

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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