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PCD FL0X

Plasmid

#12563

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Type

Empty backbone

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
C054

Plasmid

#174342

Purpose

Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Firefly luciferase

Use

In vitro transcription template

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPR219.05

Plasmid

#78554

Purpose

Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output

Depositor

Jeffrey Tabor

Article

Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

Insert

uirR

Use

Synthetic Biology

Promoter

J23115

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS7

Plasmid

#32056

Purpose

Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Type

Empty backbone

Expression

Bacterial

Promoter

Ps7 promoter, originated from Paraburkholderia xe…

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRC072

Plasmid

#225982

Purpose

J23110_buj_sfGFP_buj_mRFP

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Insert

sfGFP (with buj RBS), mRFP (with buj RBS)

Expression

Bacterial

Promoter

J23110

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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DinoIII-arrO

Plasmid

#128142

Purpose

Dinoflagellate backbone for rifampin resistant codon optimized gene

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase

Expression

Bacterial

Promoter

Dinolagellate potential promoters

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRC241

Plasmid

#225981

Purpose

J23117_RC2_mTagBFP_Buj_mRFP dual operon reporter

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Insert

mTagBFP (with rc RBS), mRFP (with buj RBS)

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAM1303

Plasmid

#40243

Depositor

Susan Golden

Article

Kulkarni et al Mol Microbiol. 1997 Jun;24(6):1131-42.

Insert

Sp/Sm Resistance, neutral site I integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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DinoIII-arrO-N

Plasmid

#128143

Purpose

Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase + potential promoter

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJK504

Plasmid

#155381

Purpose

dCas12a crRNAs

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRC055

Plasmid

#225977

Purpose

dRxCas13d expressed from pTet promoter

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Insert

dRxCas13d

Use

CRISPR

Promoter

pTet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19_BAS1509

Plasmid

#82354

Purpose

T7 in vitro transcription template for Bas mRNA

Depositor

Nils Walter

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;11

Insert

BAS1509 hypothetical protein

Use

Unspecified

Promoter

T7

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pVHD

Plasmid

#61303

Purpose

Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

Type

Empty backbone

Tags

HA

Expression

Bacterial

Promoter

PV10

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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DHFR

Plasmid

#64200

Purpose

Expression of E. coli DHFR

Depositor

Vern Schramm

Article

Wang et al J Am Chem Soc. 2014 Jun 11;136(23):8333-

Insert

Dihydrofolate reductase

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pUC57-Rluc-let-7-A114-N40

Plasmid

#50563

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-let7-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLNL1130

Plasmid

#160861

Purpose

PJ23110-RBS-AbWS/DGAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

O-acyltransferase WSD

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJP_Ctrl10

Plasmid

#219672

Purpose

Contains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Inserts

mCherry
lacI

Promoter

PT3lacO and pR

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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