We narrowed to 3,301 results for: actin

1. 5HT6-YFP-ThymosinBeta4(KK18,19EE)

   Plasmid

   #96807

   Purpose

   non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   KK18,19EE prevents G-actin binding

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Rab7a-7

   Plasmid

   #56443

   Purpose

   Localization: Ras GTPase, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N117S in NM_004637.5

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pODN-mR2-ACTB

   Plasmid

   #183868

   Purpose

   Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB homology arms with mRuby2-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. plko-shmfn2-mfn2-Rescue

   Plasmid

   #160655

   Purpose

   "Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   mfn2 (MFN2 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

   Plasmid

   #174631

   Purpose

   Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Tags

   GFP-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR201-Eps8L2

   Plasmid

   #196526

   Purpose

   Entry vector for Gateway with Eps8L2

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2-FL (EPS8L2 Human)
   

   Expression

   Bacterial

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag Intersectin Short (human)

   Plasmid

   #47392

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   Intersectin short (ITSN1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCS2.0 Tag-RFP-T-UtrCH

   Plasmid

   #101279

   Purpose

   Life imaging pCS2.0 Tag-RFP-T_UtrCH

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   Utrophin (UTRN Human)
   

   Tags

   TagRFP-T

   Expression

   Mammalian

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-BASIC-ARHGAP29 PROMOTER

   Plasmid

   #104155

   Purpose

   luciferase reporter with ARHGAP29 promoter

   Depositor

   Marius Sudol

   Article

   Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

   Insert

   ARHGAP29 promoter (ARHGAP29 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   ARHGAP29

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid

    #174633

    Purpose

    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Tags

    mCherry-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMYs-MFN2-IRES-mitoRFP

    Plasmid

    #121997

    Purpose

    Expression of MFN2 and mitoRFP in mammalial cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    MFN2-IRES-mitoRFP (MFN2 Human)
    

    Use

    Retroviral

    Mutation

    silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ps-3HA-Beta2-PSCFP2-N

    Plasmid

    #114186

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Tags

    3HA and PS-CFP2

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid

    #174634

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Tags

    mCherry-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid

    #131138

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and tdTomato

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-CLCb (EED/QQN)

    Plasmid

    #47422

    Depositor

    Peter McPherson

    Article

    Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

    Insert

    CLCb EED/QQN (CLTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EED changed to QQN, in aa20-41 region

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Nup98-pDONR221

    Plasmid

    #124241

    Purpose

    Gateway entry clone of Drosophila Nup98-96 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Nup98-96 (Nup98-96 Fly)
    

    Expression

    Insect

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR CMVie-Lifeact-iRFP670 L1-R5

    Plasmid

    #84390

    Purpose

    Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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