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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

ADE3p

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 S1351R

Plasmid

#21941

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa1209-1362 only, S1351R

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 D1346N

Plasmid

#21940

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, D1346N

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFL-TK-AID3'UTR

Plasmid

#19986

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

Plasmid

#72233

Purpose

Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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M71::Myc6-IRES-taulLacZ LNL TV

Plasmid

#15619

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::Myc6-IRES-taulLacZ LNL TV (Myc Mouse)

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pET22b-OleTLA

Plasmid

#251243

Purpose

Expression of CYP152-OleTLA

Depositor

Pimchai Chaiyen

Article

Phaisan et al J Biol Chem. 2025 Jul;301(7):110397. doi

Insert

OleTLA

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER1-TF1-dsRED

Plasmid

#213044

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER2-TF1-dsRED

Plasmid

#213045

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLoxP-EGFP-crRNA-entry

Plasmid

#213048

Purpose

SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

LoxP-EGFP-MALAT1-triplex

Use

CRISPR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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L1p1_min35S_Luciferase

Plasmid

#231035

Purpose

Cloning vector to clone promoter fragment in front of minimal p35S:Luciferase:tNOS

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Minimal p35S:Luciferase:tNOS

Use

Luciferase

Expression

Bacterial

Promoter

minimal p35S

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-C+F-GFP

Plasmid

#209699

Purpose

Expresses GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

GFP

Use

AAV

Promoter

CBA

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM_Δflv3.sm

Plasmid

#185533

Purpose

a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

aadA gene flanked by flv3 upstream and downstream regions

Use

Synthetic Biology

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IRdist_scram

Plasmid

#162319

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

The distal Upstream Motif site is scrambled

Promoter

pBAD

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H10L

Plasmid

#198683

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H10R

Plasmid

#198684

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H09R

Plasmid

#198681

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H09L

Plasmid

#198682

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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