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Plasmid#99698PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAVDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only
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pJKR-H-benM
Plasmid#73925PurposeMuconate biosensor. Expresses GFP proportional to muconate concentration within the cell.DepositorInsertmuconate biosensing apparatus
UseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKAR5
Plasmid#149461PurposeBroad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertAraC-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLlR5
Plasmid#149464PurposeBroad-Host range expression vector, RK2 and pUC origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRR5
Plasmid#149468PurposeBroad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertRhaS-RhaR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_53
Plasmid#109251PurposepUC BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5406
Plasmid#132661PurposeRSF1010-based broad host-range plasmid with additional RK2 bom site and pUC originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKCiR5
Plasmid#149462PurposeBroad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDO23A
Plasmid#27367DepositorInsertpUC/ori, bla
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLxR5
Plasmid#149465PurposeBroad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-Puro-ccdB
Plasmid#82580PurposeA modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassetteDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCBhAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPA3
Plasmid#24777DepositorTypeEmpty backboneUseIntegrating vectorTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKCyR5
Plasmid#149463PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCM121
Plasmid#220833PurposeEryA sensor with increased sensitivity (mphA and mphR (T17R) mutation) and high level resistance (ermC)DepositorInsertmphR
UseTagsExpressionMutationT17RPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILxR5
Plasmid#149497PurposeBroad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCyGe2
Plasmid#167515PurposeBroad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.DepositorInsertCymRAM-GmR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3.-6_human ProCas9Flavi-6
Plasmid#121630PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKCyR6
Plasmid#149493PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK421
Plasmid#114688PurposeRFP-Nano tagging at NuMA's C-terminal locusDepositorInsertRFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only