We narrowed to 13,472 results for: sequence
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Plasmid#216741PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [INIGGALQ…Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgCTG-CMV-GFP
Plasmid#216734PurposeAAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.DepositorArticleInsertsgCTG
UseAAV and CRISPRExpressionMammalianPromoterU6 and CMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-6xTRE-FLEX-hM4Di.mCherry
Plasmid#169415PurposeActivity-dependent, cre-dependent, temporally restricted inhibitory DREADD expressionDepositorInserthM4Di.mCherry
UseAAVTagsmCherryMutationhM4Di is Gi-coupled DREADD.Derived from human mus…PromoterMinimal TATAAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
Plasmid#207135PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-inDepositorInsert5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
ExpressionMammalianAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b-His_RAD23A-UBA2
Plasmid#201550PurposeExpresses UBA2 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
Tags6x HisExpressionBacterialMutationExpresses amino acids 311-363 of DNA polymerase i…PromoterT7Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HypaR-SpCas9
Plasmid#126764PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.DepositorInsertB-Hypa2SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationR661A; N692A; M694A; Q695A; H698A; amino acids 10…PromoterCBhAvailable SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor_50-83-YMVLtoAADD_AB
Plasmid#148376PurposeInsect Expression of DmThor_50-83-YMVLtoAADDDepositorInsertDmThor_50-83-YMVLtoAADD (Thor Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence giv…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-V75DL79D_AB
Plasmid#148377PurposeInsect Expression of DmThor-V75DL79DDepositorInsertDmThor-V75DL79D (Thor Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence giv…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmThor-S65DT70D_AB
Plasmid#148375PurposeInsect Expression of DmThor-S65DT70DDepositorInsertDmThor-S65DT70D (Thor Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence giv…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-del76-84-Y54AM59A_Z
Plasmid#148191PurposeBacterial Expression of DmThor-del76-84-Y54AM59ADepositorInsertDmThor-del76-84-Y54AM59A (Thor Fly)
ExpressionBacterialMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-del76-84-V5His6_Z
Plasmid#148152PurposeInsect Expression of DmThor-del76-84DepositorInsertDmThor-del76-84 (Thor Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X
Plasmid#147983PurposeInsect Expression of DmNot1_1-1717-del1156-1169DepositorInsertDmNot1_1-1717-del1156-1169 (Not1 Fly)
ExpressionInsectMutation6 silent mutations and two non silent N605S, K759…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X
Plasmid#147984PurposeInsect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204ADepositorInsertDmNot1_1-1717-del1156-1169-R1197AN1203AF1204A (Not1 Fly)
ExpressionInsectMutation6 silent mutations and two non silent N605S, K759…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X
Plasmid#147985PurposeInsect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEADepositorInsertDmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)
ExpressionInsectMutation6 silent mutations and two non silent N605S, K759…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_X
Plasmid#147990PurposeInsect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAresDepositorInsertDmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
ExpressionInsectMutation8 silent mutations and two non silent N605S, K759…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X
Plasmid#147991PurposeInsect Expression of DmNot1-del1156-1169-dsRNAresDepositorInsertDmNot1-del1156-1169-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and two non silent N605S, K75…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147875PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and two non silent N605S, K75…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147859PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and two non silent N605S, K75…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsEDC4-del974-1265_V
Plasmid#147786PurposeMammalian Expression of HsEDC4-del974-1265DepositorInsertHsEDC4-del974-1265 (EDC4 Human)
ExpressionMammalianMutationone non silent mutation I286T compared to the seq…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-del76-85-V5His6_T
Plasmid#147613PurposeInsect Expression of DmThor-del76-85DepositorInsertDmThor-del76-85 (Thor Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only