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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Monosiga brevicollis CBS-pair domain

Plasmid

#166435

Purpose

Express pGEX-6P-1 Monosiga brevicollis CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Monosiga brevicollis CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Naegleria fowleri CBS-pair domain

Plasmid

#166437

Purpose

Express pGEX-6P-1 Naegleria fowleri CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Naegleria fowleri CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Toxoplasma gondii CBS-pair domain

Plasmid

#166438

Purpose

Express pGEX-6P-1 Toxoplasma gondii CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Toxoplasma gondii CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIALD2E

Plasmid

#185866

Purpose

Knocking out ALD6

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD(-125, 40)- ALD6(1054, 1749)

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTL335

Plasmid

#168397

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP24

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTL336

Plasmid

#168396

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP34

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0033

Plasmid

#185624

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-2

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H

Plasmid

#187188

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

D34R, D87R

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H

Plasmid

#187184

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

L32F, L33F

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H

Plasmid

#187185

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90A, V91A

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H

Plasmid

#187183

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

L32A, L33A

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox12-PLAT-EGFP-6H

Plasmid

#187190

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000069463 (alox12 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-PKCgamma-C2-EGFP-6H

Plasmid

#187115

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000126583 (PRKCG Human)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0032

Plasmid

#185623

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-1

Use

CRISPR and Synthetic Biology

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

Plasmid

#187187

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

D34K, D87K

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNB202

Plasmid

#169113

Purpose

TESEC expression vector for mEGFP

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2351_Tier3(SB)-Zeo

Plasmid

#169648

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven ZeoR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MD-OCT4

Plasmid

#115691

Purpose

Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Article

Friman et al Elife. 2019 Dec 3;8. pii: 50087. doi: 10

Insert

SNAP-MD-OCT4

Use

Lentiviral

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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