168,234 results

1. p5E-3x125bp-mnx1

   Plasmid

   #169043

   Purpose

   5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   Mnx1 enhancer (3x) (mnx1 Zebrafish)
   

   Use

   Gateway entry cloning vector

   Promoter

   3xMnx1

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EcMscL

   Plasmid

   #92418

   Purpose

   E. coli Mechanosensitive Channel of Large Conductance

   Depositor

   Douglas Rees

   Insert

   MscL (mscL E. coli)
   

   Tags

   6x Histidine Tag and Thrombin Cleavage Site betwe…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-FLAG-Tev_ACTB (beta-actin)

   Plasmid

   #188453

   Purpose

   Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   ACTB (ACTB Human)
   

   Tags

   6xHis-FLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT GBA pcDNA3.1

   Plasmid

   #188580

   Purpose

   Ectopic expression of wild-type GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGC-1 alpha promoter luciferase delta CRE

   Plasmid

   #8888

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the CREB bind…

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES-EGFP-puro

   Plasmid

   #45567

   Depositor

   Michael McVoy

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   IRES-EGFP-Puro

   Expression

   Mammalian

   Promoter

   Phcmv (strong human cytomegalovirus immediate ear…

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRB-mCherry-FTH1

   Plasmid

   #100749

   Purpose

   Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK002 pME mCerulean

   Plasmid

   #195956

   Purpose

   Gateway pME fluorophore

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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