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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,790 results for: son

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Showing: 10861 - 10880 of 31790 results

  1. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

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  11. CMV+ plasmid with onboard mCherry cassette

    Plasmid
    #169741
    Purpose
    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169741-plasmid-map-sequence-id-334213

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  15. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162684-plasmid-map-sequence-id-321526

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  17. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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  20. pEBTet-p52-HDM2_sensor

    Plasmid
    #127184
    Purpose
    Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction
    Depositor
    Kai Johnsson
    Publication
    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
    Tags
    HisTag and StrepTag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    June 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127184-plasmid-map-sequence-id-246982

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Showing: 10861 - 10880 of 31790 results