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pQCXIP EGFP-ER [TM(SAC1)]

Plasmid

#186475

Purpose

Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Publication

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-hsChRmine-oScarlet-WPRE

Plasmid

#183526

Purpose

Optogenetics

Depositor

Karl Deisseroth

Publication

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet

Use

AAV

Mutation

H33R

Promoter

Ef1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMC234r-ccdB/TPK2

Plasmid

#176179

Purpose

yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

dropout cassette containing toxic genes ccdB and TPK2

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-mGFP-APEX2

Plasmid

#171936

Purpose

Reporter for correlated light and electron microscopy.

Depositor

David Berson

Publication

Stedehouder et al Elife. 2019 Nov 19;8. pii: 48615. doi: 1

Insert

mGFP-APEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmFbxw7_R505C

Plasmid

#160104

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

R505C

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgSIK2

Plasmid

#138708

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-TagBFP

Plasmid

#117792

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cre

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cre

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCB32

Plasmid

#111911

Purpose

Expresses gRNA to target Nte1 locus and has HygR marker

Depositor

Yi Tang

Insert

gRNA cassette targeting Nte1 locus

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

SNR52p

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-EGFP

Plasmid

#66821

Purpose

dCas9 fused to BFP and EGFP

Depositor

Yonglun Luo

Publication

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-EGFP

Tags

dSpCas9-BFP-EGFP

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Human USP30 (64-502, construct 8, MG-26-21)

Plasmid

#110745

Purpose

Bacterial expression of human USP30 (construct 8)

Depositor

David Komander

Publication

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

F348D, M350D, I353E + insertion deletion: 64-357;…

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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DPYSL4 (DPYSL4A-c003)

Plasmid

#110263

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DPYSL4 (DPYSL4 Human)

Tags

His6-ZBasic-TEV

Expression

Insect

Promoter

polyhedrin promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Publication

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-WPRE

Plasmid

#87886

Purpose

To produce Lentiviral vector for gene editing

Depositor

Nicole Déglon

Publication

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

hCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

mouse PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE

Plasmid

#87912

Purpose

Kamicas9 lentiviral vector

Depositor

Nicole Déglon

Publication

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

shHTT1, sgCas9, cherry

Use

Lentiviral

Promoter

H1, 7SK, PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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DAPK1_HUMAN_D0

Plasmid

#79728

Purpose

This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK1 (DAPK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only