We narrowed to 90,070 results for: Mal
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Plasmid#169622PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2317
Plasmid#169624PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182682PurposeContains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.DepositorInsertmouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterNAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
TKIT NFL gRNA1 gRNA2
Plasmid#182681PurposeExpression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.DepositorInserttwo Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
UseCRISPRExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182685PurposeEncodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.DepositorInsertsExpressionMammalianPromoterCMV and NAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-H2BC11-TagBFP
Plasmid#183867PurposeRepair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.DepositorInsertH2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mTagBFPExpressionMammalianMutationHomology arms contain point mutations to remove t…Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-ECT2_sgRNA
Plasmid#183875PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1-Armc12/FLAG
Plasmid#180494PurposeExpression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
TagsFLAG tagExpressionMammalianPromoterCAG promoterAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Lrrc23-FLAG
Plasmid#179921PurposeVector backbone. pClgn-promoter driven expression.DepositorInsertLrrc23 (Lrrc23 Mouse)
TagsFLAGExpressionMammalianMutationStop codon removedPromoterCalmeginAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Lrrc23-1D4
Plasmid#179922PurposeVector backbone. pClgn-promoter driven expression.DepositorInsertLrrc23 (Lrrc23 Mouse)
Tags1D4ExpressionMammalianMutationStop codon removedPromoterCalmeginAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9
Plasmid#163550PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…Available SinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)
Plasmid#155006PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/P1424Q/Y1426S; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)
Plasmid#155313PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationM1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)
Plasmid#155312PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationM1423R/P1424V/Y1426H; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)
Plasmid#155007PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA1419R/P1424Q/Y1426S; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)
Plasmid#155008PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationS1421R/P1424Q/Y1426S; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)
Plasmid#155009PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)
Plasmid#154977PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424/Y1425I/Y1426I; insert codes for EBA175 resi…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)
Plasmid#154875PurposeExpresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1DepositorInsertPfBAEBL (PF3D7_0506900 P. falciparum)
TagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationI1134V, P1135L, Y1136V, F1137V; insert codes for …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g3 (BB24)
Plasmid#139459PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only