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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,015 results for: flag

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Showing: 10941 - 10960 of 15015 results

  2. pCDNA5_C12orf49-miniTurbo

    Plasmid
    #155112
    Purpose
    transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID
    Depositor
    Jason Moffat
    Publication
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C12orf49 (SPRING1 Human)
    Tags
    3x Flag and miniTurbo
    Expression
    Mammalian
    Promoter
    CMV/TO inducible promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    155112-plasmid-map-sequence-id-308052

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  6. pEBTetD-SNAP-Centrin2

    Plasmid
    #136785
    Purpose
    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Centrin2 (CETN2 Synthetic, Human)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    136785-plasmid-map-sequence-id-276017

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  8. pEBTet-SNAP-Cep290

    Plasmid
    #136804
    Purpose
    Mammalian expression of the centrosomal protein Cep290 N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Cep290 (CEP290 Synthetic, Human)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136804-plasmid-map-sequence-id-275879

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  9. pEBTet-SNAP-γ-tubulin

    Plasmid
    #136799
    Purpose
    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-TUBG (TUBG1 Synthetic, Human)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136799-plasmid-map-sequence-id-275873

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  10. pEBTet-SNAP-Cep41

    Plasmid
    #136794
    Purpose
    Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Cep41 (CEP41 Synthetic, Human)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136794-plasmid-map-sequence-id-275860

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  13. BII-gR-BtW-eSpCas9

    Plasmid
    #133358
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Publication
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9
    Tags
    Flag-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133358-plasmid-map-sequence-id-269429

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  15. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3xFLAG and 126aa domain from HSV-1 UL12 fused to …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  16. pcDNA3.1(+)- e18 LOF

    Plasmid
    #131729
    Purpose
    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")
    Depositor
    Alberto Ciccia
    Publication
    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    Tags
    FLAG and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    131729-plasmid-map-sequence-id-266392

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  17. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

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  18. pCAG-T3-eSpCAS9-NG-pA

    Plasmid
    #131466
    Purpose
    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized eSpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131466-plasmid-map-sequence-id-262445

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  19. pCAD108_Arg273His_pCMV6Entry-hIRG1

    Plasmid
    #124878
    Purpose
    Expresses hCAD Arg273His mutant in mammalian cells
    Depositor
    Konrad Buessow
    Publication
    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cis-aconitate decarboxylase (ACOD1 Human)
    Tags
    myc and FLAG tags
    Expression
    Mammalian
    Mutation
    mutated Arg273 to His
    Promoter
    CMV
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124878-plasmid-map-sequence-id-257458

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Showing: 10941 - 10960 of 15015 results