We narrowed to 6,747 results for: &alpha

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PBS/pU6-HIF1 alpha RNAi plasmid 2

Plasmid

#21104

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

RNAi against hypoxia inducible factor 1alpha

Expression

Mammalian

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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hum alpha ENAC promoter a25-a23

Plasmid

#83427

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

Insert

human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)

Use

Luciferase

Tags

Luciferase

Promoter

alpha ENAC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

Plasmid

#136603

Purpose

Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

FKBP-C-mSOG-J-alpha

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

Plasmid

#117662

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT1)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

Plasmid

#225433

Purpose

Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

4xCBS:mDFR:Rep/RepA:Tnos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

Plasmid

#160633

Purpose

TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31-RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Danio alpha A-crystallin 1kb promoter/AcGFP

Plasmid

#98096

Purpose

aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha A-crystallin 1 kb fragment (-1028/-1)

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)

Plasmid

#52216

Purpose

mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation

Depositor

Eric Huang

Article

Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

Insert

HIF1alpha (401delta603) LCLL (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

aa 401-603 deleted, removes the oxygen-dependent …

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204343

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

Plasmid

#211839

Purpose

Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

R181A R185A

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV MP-135 mRFP-3MREs (AAV-alpha)

Plasmid

#206929

Purpose

Alpha cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Insert

mRFP

Use

AAV

Expression

Mammalian

Promoter

MP-135

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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V308 pDNR MCS lacZ alpha - Donor

Plasmid

#11691

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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Venus Cam KII alpha mutant T286D

Plasmid

#29429

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

T286D mutation in CamKIIa sequence

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta LIM

Plasmid

#40933

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

LIM domain deleted- amino acids 221-295

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha delta C-term

Plasmid

#40930

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 296-600

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only