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pET28-fbaA

Plasmid

#252953

Purpose

Expresses fructose bisphosphate aldolase in E. coli

Depositor

David Kwan

Article

Ghosh et al bioRxiv 2025.06.15.659766

Insert

fructose bisphosphate aldolase (fbaA )

Tags

His-tagged

Expression

Bacterial

Promoter

T7

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1P10A-Myc-MBP-HA

Plasmid

#228943

Purpose

Expression AtTIR1P0A-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1P10A (TIR1 Mustard Weed)

Tags

myc

Expression

Bacterial

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1-Myc-MBP-HA

Plasmid

#222709

Purpose

Expression AtTIR1-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1 (TIR1 Mustard Weed)

Tags

myc

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPCaspase

Plasmid

#214042

Purpose

Bacterial expression plasmid for PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-C-term

Plasmid

#214045

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 154-666

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Luciferase-CD4-bla

Plasmid

#189985

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCX:cIPAD-3 (full length)

Plasmid

#208520

Purpose

ddGFP-A and ddGFP-B3-inserted Pcdhα4

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Tags

HA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:cIPAD-1 (full length)

Plasmid

#208519

Purpose

ddGFP-A and ddGFP-B1-inserted Pcdhα4

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Tags

HA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiPOLR2D

Plasmid

#202445

Purpose

Expression of a CRISPRi guide targeting POLR2D

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

POLR2D gRNA (POLR2D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2(3)-C911

Plasmid

#202452

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2(3)

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgUBR4

Plasmid

#202442

Purpose

Expression of a guide RNA targeting UBR4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBR4 gRNA (UBR4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2-C911

Plasmid

#202450

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSLiP-G1G2

Plasmid

#188965

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pA-RFP-rG2

Plasmid

#188969

Purpose

IPTG inducible mCherry with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

mCherry
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRAF-MutL(R95F)

Plasmid

#86608

Purpose

E. coli Mutl(R95F) expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL(R95F)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

R95F

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBW301ParsR-Amp30T

Plasmid

#78653

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
csr-1_frq[mCherry_V5H6]

Plasmid

#245517

Purpose

Epitopically express mCherry-tagged FRQ driven by frq endogenous promoter at csr-1 locus.

Depositor

Jay C Dunlap

Article

Wang et al J Cell Biol. 2026 Jun 1;225(6):e20250904

Insert

frq

Use

Neurospora expression

Tags

V5H6 and mCherry

Promoter

frq promoter

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pREW2[luciferase fragment in pPD129.36/L4440]

Plasmid

#253297

Purpose

In HT115 host, expresses dsRNA corresponding to a luciferase sequence with no matches in the C. elegans genome. Excellent for negative control bacterially mediated RNAi

Depositor

David Reiner

Article

Shin et al Cell Rep. 2018 Sep 4;24(10):2669-2681.e5

Insert

luciferase fragment

Expression

Bacterial

Promoter

Twin T7 promoters

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pET28-SpBglA-2

Plasmid

#252910

Purpose

Expresses 6-phosphoglusidase in E.coli

Depositor

David Kwan

Article

Ghosh et al bioRxiv 2025.06.15.659766

Insert

6-phospho-beta-glucosidase

Tags

His-tagged

Expression

Bacterial

Promoter

T7

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-ΔHsFBXO28-Myc-MBP-HA

Plasmid

#228946

Purpose

Expression ΔHsFBXO28-Myc (lacking F-box domain) and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

ΔHsFBXO28 (FBXO28 Human)

Tags

myc

Expression

Bacterial

Mutation

deletion of the F-box domain

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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