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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,346 results for: D10

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Showing: 1081 - 1100 of 2346 results

  4. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  5. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  6. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  8. pAAV2-CAGIG-LAMP1-mGL

    Plasmid
    #222394
    Purpose
    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.
    Depositor
    Pantelis Tsoulfas
    Insert
    LAMP1 and mGreenlantern (LAMP1 Human, Homo Sapiens, Synthetic)
    Use
    AAV
    Tags
    LAMP1 and mGreenLantern
    Expression
    Mammalian
    Promoter
    CAG or CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222394-plasmid-map-sequence-id-446519

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  16. pE2F3.3.1-gDNA

    Plasmid
    #113776
    Purpose
    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3
    Depositor
    Kevin White
    Insert
    E2F3 (E2F3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113776-plasmid-map-sequence-id-230565

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Showing: 1081 - 1100 of 2346 results