We narrowed to 12,192 results for: HAL;

1. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139485

   Purpose

   PCR template for Halo-Tag gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   HaloTag-loxP-P2A-dTKneo-pA-loxP
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

   Plasmid

   #198599

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-143-144
   

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

   Plasmid

   #134612

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 375 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)

   Plasmid

   #215001

   Purpose

   Shuttling vector for a mitophagy reporter

   Depositor

   Wade Harper

   Insert

   pSu9 (Presequence of Fo-ATPase subunit 9)
   

   Use

   Gateway

   Tags

   HaloTag(7)-mGFP

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-BLAST Halo-flag-CAD

   Plasmid

   #188117

   Purpose

   Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag and Halo tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

   Plasmid

   #87644

   Purpose

   For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   myr::SNAPf>myr::CLIPf>myr::Halo2
   

   Expression

   Insect

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHTN HaloTag CMV-neo/ERGIC2-variant

   Plasmid

   #51477

   Purpose

   Expresses ERGIC2-variant in mammalian cells, HaloTag for cellular localization

   Depositor

   Simon Kwok

   Article

   Kwok et al DNA Cell Biol. 2013 Dec 4.

   Insert

   ERGIC2 (ERGIC2 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   4 base deletion at codon #189

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. C514-E47: attB5r-Halotag-nostop-attB1r

    Plasmid

    #162963

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Halotag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

    Plasmid

    #175814

    Purpose

    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller, Mark Banfield

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Promoter

    none

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. R713-M50-303: CMV51p> Halotag-HRasHVR

    Plasmid

    #159685

    Purpose

    Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-HRasHVR (HRAS Human)
    

    Tags

    Halotag

    Expression

    Mammalian

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. R713-M48-303: CMV51p> Halotag-Kras4aHVR

    Plasmid

    #159684

    Purpose

    Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Kras4aHVR (KRAS Human)
    

    Tags

    Halotag

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. R703-M80-303: CMV51p> Halotag-Kras4bHVR

    Plasmid

    #159683

    Purpose

    Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Kras4bHVR (KRAS Human)
    

    Tags

    Halotag

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. R713-M52-303: CMV51p> Halotag-NRasHVR

    Plasmid

    #159686

    Purpose

    Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-NRasHVR (NRAS Human)
    

    Tags

    Halotag

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

    Plasmid

    #69907

    Purpose

    Expresses a circular RNA containing the 5' half of GFP in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    GFP, ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid

    #74603

    Purpose

    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    SFFV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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