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pFOSGE_DUM1H01

Plasmid

#71090

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

nej (nej Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
186_pETcon_SARS2_XBB1-5

Plasmid

#208086

Purpose

yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 XBB.1.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-1

Plasmid

#86387

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter

Use

Stap-seq spike in vector

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-3

Plasmid

#86389

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1

Use

Stap-seq spike in vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-4

Plasmid

#86390

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2

Use

Stap-seq spike in vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-2

Plasmid

#86388

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter

Use

Stap-seq spike in vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H09

Plasmid

#71003

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

SNF4Agamma (SNF4Agamma Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C07

Plasmid

#70985

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

14-3-3zeta (14-3-3zeta Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2B09

Plasmid

#70918

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

gro (gro Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C03

Plasmid

#70959

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf4 (Taf4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLEGFP-WT-STAT3

Plasmid

#71450

Purpose

Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

S727A

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLEGFP-K685R-STAT3

Plasmid

#71449

Purpose

Retroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

K685R

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pLEGFP-K49R-STAT3

Plasmid

#71447

Purpose

Retroviral expression of K49R-STAT3. Please note that this plasmid contains K49R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

K49R

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pEBB HA CCDC22

Plasmid

#74903

Purpose

Express CCDC22 in mammalian cells

Depositor

Ezra Burstein

Article

Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

Insert

CCDC22 (CCDC22 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLEGFP-K140R-STAT3

Plasmid

#71448

Purpose

Retroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

K140R

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Alt AlphaPS3

Plasmid

#14070

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Alt Alpha PS3 Integrin (scb Fly)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3H04

Plasmid

#70971

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf6 (Taf6 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only