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pPromE8 (GB0914)

Plasmid

#68175

Purpose

Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pEIIIB

Plasmid

#14060

Depositor

Richard Hynes

Publication

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIB (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers were attached to HindIII - HinfI fr…

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pCD013

Plasmid

#252943

Purpose

Label Lactiplantibacillus plantarum with sfGFP (erythromycin-resistant)

Depositor

Caroline Ajo-Franklin

Publication

Li et al Nat Biotechnol (2026).

Insert

sfGFP

Expression

Bacterial

Promoter

Ptuf

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pMB1-A-pBAD-dCas9

Plasmid

#190132

Purpose

Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9

Use

CRISPR

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-probe2

Plasmid

#105455

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Publication

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISH-Trpc2-probe1

Plasmid

#105473

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Publication

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Trpc2 (Trpc2 Mouse)

Use

To ganerate riboprobe

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISH-Tshz2

Plasmid

#74361

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz2. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 2 (Tshz2 Mouse)

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGUS (GB0208)

Plasmid

#68203

Purpose

Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

GUS coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRB7-6

Plasmid

#24700

Depositor

George Allen

Publication

Allen et al Plant Cell. 1996 May . 8(5):899-913.

Insert

Tobacco Rb7 MAR (SAR)

Use

E. coli phagemid

Available since

July 8, 2010

Availability

Academic Institutions and Nonprofits only
M10.2 Southern Blot 5'-external probe

Plasmid

#15861

Depositor

Peter Mombaerts

Publication

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

M10.2

Expression

Bacterial

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Olfr78

Plasmid

#105491

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Publication

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Olfr78 (Or51e2, MOR18-2 Mouse)

Use

To ganerate riboprobe

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFKBPmCherry-Akt2(K14A)

Plasmid

#86597

Purpose

Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBB-mCherry-Akt2(K14A) (AKT2 )

Expression

Mammalian

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHcPro (GB0067)

Plasmid

#68196

Purpose

Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

TEV HcPro coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMOR10-ISH

Plasmid

#15857

Depositor

Peter Mombaerts

Publication

Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

Insert

MOR10 (Or4e1 Mouse)

Use

For riboprobe, in situ hybridization

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pME-FastFUCCI (JDW 1488)

Plasmid

#242556

Purpose

Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Use

Gateway Cloning

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)

Plasmid

#86595

Purpose

Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt2(K14A/R25E) (AKT2 )

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.b

Plasmid

#66588

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Publication

Li et al Genome Biol. 2015 May 28;16(1):111.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Pten deletion (Pten Mouse)

Expression

Mammalian

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pDS68

Plasmid

#48366

Purpose

GFP-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Publication

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

GFP-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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