We narrowed to 5,840 results for: pcas

1. pCAGGS-FLAG-mISG15

   Plasmid

   #12443

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Myr-p110-IH

   Plasmid

   #15689

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Tags

   Myr

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pX330_CALR sgRNA / hSpCas9

   Plasmid

   #172838

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-VPS15-TSF

   Plasmid

   #203563

   Purpose

   Mammalian expression of VPS15 with TwinStrep-Flag tag

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   VPS15 (PIK3R4 Human)
   

   Tags

   TwinStrep Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tubp-Gal80 in pCaSpeR4

   Plasmid

   #17748

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Tags

   SV40 polyA and Tubulin 1alpha promoter

   Expression

   Insect

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRha-ABE8e-SpCas9-NG

   Plasmid

   #201190

   Purpose

   Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

   Depositor

   Jonathan Yen

   Article

   Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

   Insert

   ABE8e-SpCas9-NG
   

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG mito-mTagBFP2

   Plasmid

   #105011

   Purpose

   Mitochondrial marker

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-PIF3-mEGFP

   Plasmid

   #100283

   Purpose

   Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N-terminus (1-100 aa) of PIF3 is used

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG_Xph20-GCaMP7f-CCR5TC

   Plasmid

   #216534

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Tags

   GCaMP7f

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-OSF-KATNA1

    Plasmid

    #160051

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG roGFP2-mito

    Plasmid

    #168509

    Purpose

    Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

    Insert

    mito-roGFP2
    

    Tags

    roGFP2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG2-EGFP-C-IFT140

    Plasmid

    #218739

    Purpose

    Expresses C-terminally EGFP-tagged IFT140 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT140 (IFT140 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-HF1-plus

    Plasmid

    #126768

    Purpose

    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-sp-flag-hSema4D

    Plasmid

    #190656

    Purpose

    Expresses Human Sema4D with N terminal flag after signal peptide

    Depositor

    Victor Tarabykin, Andrew Newman

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4D (SEMA4D Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_Xph20-mEos3.2-CCR5TC

    Plasmid

    #135532

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-SpCas9-HF1

    Plasmid

    #108301

    Purpose

    pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-eNpHR3.0-mRuby2-ST

    Plasmid

    #109134

    Purpose

    Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eNpHR3.0-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-R2Tg-3xFlag

    Plasmid

    #223250

    Purpose

    Mammalian expression of C-terminally Flag-tagged R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-HiBiT-CD81

    Plasmid

    #162593

    Purpose

    N-terminally HiBiT-tagged human CD81 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD81 (CD81 human, Synthetic, Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GFP-Rab27a

    Plasmid

    #194985

    Purpose

    overexpress Rab27a

    Depositor

    Angelique Bordey

    Insert

    GFP-Rab27a
    

    Expression

    Bacterial

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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