We narrowed to 11,549 results for: ANG

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CPH3509 (YCp LEU2 Rpb1 T1471A)

Plasmid

#91812

Purpose

Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed threonine 1471 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3511 (YCp LEU2 Rpb1 P1472A)

Plasmid

#91813

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1472 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

Plasmid

#91818

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1355 and lysine 1435 to alanines; …

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g3 (BB24)

Plasmid

#139459

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EC71102

Plasmid

#154065

Purpose

Level 0 Golden Gate vector; Unmodifed CRE

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE

Use

Synthetic Biology; Standard cloning vector used b…

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2(732)

Plasmid

#154099

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-T92Q-Fc(IgA1)

Plasmid

#145162

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v4-Fc(IgA1)

Plasmid

#145159

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v6

Plasmid

#145153

Purpose

Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 6

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B Q116P G120

Plasmid

#129291

Purpose

Gateway entry clone encoding human deconjugation-resistant LC3B

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway Cloning

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 G40N-P2A-Blast

Plasmid

#123321

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLL5 sgRNA(MS2)-MS2-hA3a D131A

Plasmid

#112131

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131A

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131A mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131A mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

Plasmid

#99743

Purpose

Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Tags

myc-myc-his

Expression

Mammalian

Mutation

Changed both L165 and E166 to Alanine

Promoter

CMV

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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