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pUAST-SIK3 T196E

Plasmid

#52975

Purpose

insect expression of SIK3 T196E constitutively active mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196E constitutively active mutant

Promoter

Hsp70

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

Plasmid

#191690

Purpose

For knocking p2a-EGFP into OTX2 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

OTX2 homology arms and p2a-EGFP

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA

Plasmid

#191683

Purpose

For knocking p2a-tdTomato into LMX1A locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

LMX1A homology arms and p2a-tdTomato

Expression

Mammalian

Mutation

WT

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Bnip3L RNAi pSuper-retro

Plasmid

#17468

Depositor

Wafik El-Deiry

Article

Fei et al Cancer Cell. 2004 Dec . 6(6):597-609.

Insert

Bnip3L RNAi (BNIP3L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pCDH-PD-L1 3SA/4NQ

Plasmid

#121491

Purpose

Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1.1

Plasmid

#208020

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Expression

Bacterial

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR-eGFP (pXK-1647)

Plasmid

#208824

Purpose

The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

Depositor

Kun Xu

Article

Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMIG-Flag-RIPK1-D324A

Plasmid

#102678

Purpose

retroviral expression plasmid for RIPK1-D324A

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

Flag and IRES-GFP

Expression

Mammalian

Mutation

CHANGE Glutamate 324 to Alanine

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct9

Plasmid

#28202

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-193 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
PTPN13_PDZ_1

Plasmid

#103919

Purpose

Protein expression and purification of PTN13 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_3

Plasmid

#103921

Purpose

Protein expression and purification of PTN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL421_MLL_promoter

Plasmid

#118695

Purpose

Express MLL/KMT2A promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Promoter

MLL/KMT2A promoter

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 T196A

Plasmid

#52974

Purpose

insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196A LKB1 phosphorylation defective mutant

Promoter

Hsp70

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 K70M

Plasmid

#52973

Purpose

insect expression of SIK3 K70M (kinase dead mutant)

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

K70M kinase dead mutant

Promoter

Hsp70

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Ngn1

Plasmid

#118589

Purpose

Expresses Ngn1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Ngn1 (Neurog1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-C-Kbp

Plasmid

#178013

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-C-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Pa-Kbp

Plasmid

#178014

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Pa-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Hv-Kbp

Plasmid

#178015

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Hv-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-D-Kbp

Plasmid

#178012

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-D-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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