We narrowed to 15,982 results for: LEA

1. pEMS1938

   Plasmid

   #82563

   Purpose

   High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

   Insert

   Ple261
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Available Since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDEST14-DoubleCatcher-⍺-Lock

   Plasmid

   #216286

   Purpose

   Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ⍺-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-DoubleCatcher-β-Lock

   Plasmid

   #216287

   Purpose

   Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher β-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST14-DoubleCatcher-γ-Lock

   Plasmid

   #216288

   Purpose

   Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher γ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-DoubleCatcher-δ-Lock

   Plasmid

   #216289

   Purpose

   Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher δ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST14-DoubleCatcher-ε-Lock

   Plasmid

   #216290

   Purpose

   Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ε-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECFP-DEVG-Venus

   Plasmid

   #24538

   Depositor

   Peter Sorger

   Article

   Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

   Insert

   ECFP-DEVG-Venus
   

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   DEVDR mutated to DEVG

   Promoter

   CMV

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

8. JAK3 (JAK3A-c023)

   Plasmid

   #98229

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JAK3A (JAK3 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Mutation

   pfam00069:Pkinase 822-1091

   Promoter

   polyhedrin

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6CCR (GID4)

   Plasmid

   #119960

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 116-300

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBABE-puro-NLS-LSSmKate2

    Plasmid

    #34586

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate2
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHB-2

    Plasmid

    #140955

    Purpose

    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Mutation

    R238S mutation in PhaC

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. KLHL3 (KLHL3A-c003)

    Plasmid

    #110251

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL3 (KLHL3 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF mito-cGFP (matrix)

    Plasmid

    #188904

    Purpose

    Expresses mitochondrial GFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

    Insert

    mitochondrial GFP
    

    Tags

    Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

    Expression

    Mammalian

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET26b_Nb.AT110

    Plasmid

    #135480

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. KEAP1 (KEAP1A-c029)

    Plasmid

    #110247

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KEAP1 (KEAP1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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