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pAcGHLT-B-DDB1

Plasmid

#48638

Purpose

Expresses Full Lenght DDB1 in Insect Cells (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

DDB1 (DDB1 Human)

Expression

Insect

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
Man1B1-GFP

Plasmid

#163646

Purpose

Expresses GFP tagged Man1B1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 1B member 1 (MAN1B1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NINJ1

Plasmid

#201181

Purpose

Dox-inducible expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV Tet

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A

Plasmid

#172201

Purpose

Expresses KIF5A tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAuroraA-GFP-AURKA

Plasmid

#99877

Purpose

Expression of GFP-AURKA in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Promoter

AURKA minimal

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK7

Plasmid

#122676

Purpose

Expresses mouse PCSK7 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-IC2-FL

Plasmid

#51409

Purpose

expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-Syn1-mCherry

Plasmid

#222967

Purpose

Make lentivirus to express mCherry in syn1 expressing cells

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

mCherry

Use

Lentiviral

Promoter

synapsin I

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-wt

Plasmid

#86078

Purpose

A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A WT

Plasmid

#123089

Purpose

Expresses 3xFLAG-LC3A wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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VPS29-YFP

Plasmid

#174520

Purpose

Expresses human VPS29 with a C-terminal YFP in mammalian cells

Depositor

Juan Bonifacino

Article

Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

Insert

VPS29 (VPS29 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmPB3-VSV

Plasmid

#68039

Purpose

Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Tags

VSV

Expression

Mammalian

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV human IRS-1 CAAX

Plasmid

#11238

Depositor

Ronald Kahn

Article

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Insert

IRS-1 (IRS1 Human)

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only