We narrowed to 16,515 results for: grna

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8

Plasmid

#176184

Purpose

gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8_28bp

Plasmid

#176185

Purpose

plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL9

Plasmid

#176186

Purpose

2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g3 Cas9-T2A-mCherry

Plasmid

#164252

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC008.106

Plasmid

#171141

Purpose

Expression of J106 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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sgiRFP1

Plasmid

#113972

Purpose

Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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sgiRFP2

Plasmid

#113973

Purpose

Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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sgiRFP3

Plasmid

#113974

Purpose

Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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RIB VP64 R2

Plasmid

#161494

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting R2 in the RIB1 promoter)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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RIB VP64 R3

Plasmid

#161495

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting R3 in the RIB1 promoter)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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RIB VP64 R4

Plasmid

#161496

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting R4 in the RIB1 promoter)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-Rictor-shRNA

Plasmid

#157930

Purpose

Knockdown of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor shRNA

Use

Lentiviral and RNAi

Tags

tdTomato

Promoter

mouse U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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sg1

Plasmid

#113966

Purpose

Single short guide RNA targeting GTATAGCATACATTATACG

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-REG1-3'UTR

Plasmid

#136052

Purpose

REG1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (ATTGTATCTCTGTAGTTTAAG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1 (ZC3H12A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pYMCR2_Gb_PMLTSS

Plasmid

#140285

Purpose

CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cas9

Expression

Mammalian

Promoter

CBH

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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