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MLS-mir-17-92-Mut20

Plasmid

#64095

Purpose

expresses a mutated version of mir-17-92 (mutation of miR-20 seed)

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Mutation

mutation of miR-20 seed sequence

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-chCARex

Plasmid

#87233

Purpose

bacterial expression of GST-CAR extracellular domain

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pVL45 - pPXY>>

Plasmid

#116011

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pPXY:GR-LhG4:tPXY:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK ADRB2 3'UTR

Plasmid

#19867

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

ADRB2 3'UTR (ADRB2 Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_CD2AP-1/3

Plasmid

#91398

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-1/3 (CD2AP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8-1/1

Plasmid

#91254

Purpose

Protein expression and purification of human SH3 domain construct EPS8-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8-1/1 (EPS8 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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HLH Clock Pas 1-401 VenN

Plasmid

#47353

Purpose

Clock fragment cloned and tagged with VenN

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_BAIAP2L2-1/1

Plasmid

#91393

Purpose

Protein expression and purification of human SH3 domain construct BAIAP2L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BAIAP2L2-1/1 (BAIAP2L2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pIDS-RESTmut full-length

Plasmid

#109162

Purpose

Encodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Expression

Insect

Mutation

Cys402Ser

Promoter

p10

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMextli

Plasmid

#79262

Purpose

Expresses EGFP-tagged DmMextli in S2 cells

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli (mxt NM_164598, Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 55/S1126A

Plasmid

#59825

Purpose

Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55/S1126A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal V435R

Plasmid

#47350

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

V435R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBABE-EGFP-THAP11-rescue-3xFLAG

Plasmid

#37000

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_LCK-1/1

Plasmid

#91332

Purpose

Protein expression and purification of human SH3 domain construct LCK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LCK-1/1 (LCK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L150E

Plasmid

#47346

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L150E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Tags

His6-tev

Expression

Insect

Mutation

K70A

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB10

Plasmid

#19507

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB10 (DNAJB2 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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