We narrowed to 13,693 results for: crispr cas9

1. pGES401

   Plasmid

   #190198

   Purpose

   For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SunTag-FWAgRNA-4-22aa-TET1cd

   Plasmid

   #106435

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SunTagng14aa

   Plasmid

   #106439

   Purpose

   Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. miniCGBE1-NG (pBM1182)

   Plasmid

   #140257

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_NG-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti-px330-CRBN-T1-pGK-Pur

   Plasmid

   #107382

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. miniCGBE1-VRQR (pBM1173)

   Plasmid

   #140255

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_VRQR-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-px330-CRBN-T2-pGK-Pur

   Plasmid

   #107383

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA025

   Plasmid

   #216005

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-NG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA027

   Plasmid

   #216007

   Purpose

   Fragmid fragment: (Cas protein) nearly PAM-less

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpRY_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA026

    Plasmid

    #216006

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA007

    Plasmid

    #216002

    Purpose

    Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

    Plasmid

    #117135

    Purpose

    Expression vector for Nkx2-5 and gRNA

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    CRISPR and Lentiviral

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRGEB32-CrU6-2-35S

    Plasmid

    #226706

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrU6-2
    

    Use

    CRISPR

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRGEB32-CrU6-2-UBI

    Plasmid

    #226707

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrU6-2
    

    Use

    CRISPR

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShuttle.CC9

    Plasmid

    #192931

    Purpose

    Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Promoter

    CMV

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCD175

    Plasmid

    #113316

    Purpose

    dCas9, MCP-AsiA, rpoD_F563Y

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9, MCP-T4.AsiA, rpoD_F563Y
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    F563Y

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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