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pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179074

Purpose

Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TEF2p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-nsp12

Plasmid

#168927

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-nsp12 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LLP784_SnoopCatcher-DNMT3A-CO

Plasmid

#211777

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Spy-EZH2

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RIAM(1-666)

Plasmid

#80028

Purpose

Mammalian expression of GFP-RIAM(1-666)

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

RIAM (APBB1IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-666

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLin28A-myc-T2A-tagRFP

Plasmid

#140267

Purpose

Expresses LIN28A in mammalian cells

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

LIN28A (LIN28A Human)

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp30-C1

Plasmid

#134547

Purpose

Expresses YFP-Rpp30 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp30 (RPP30 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp8

Plasmid

#168936

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-nsp8

Plasmid

#168917

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP780_SpyCatcher-KRAB-CO

Plasmid

#211773

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-KRAB

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSMP-SUZ12_1

Plasmid

#36390

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SUZ12 (SUZ12 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pIRESneo-Ago2

Plasmid

#215841

Purpose

Expression of AGO2

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human AGO2 (AGO2 Human)

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.2

Plasmid

#190796

Purpose

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pWPT-mCherry

Plasmid

#190605

Purpose

Obtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Mutation

EGFP Kozak sequence has been replaced with a stre…

Promoter

EIF1-short

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pWzyE-distrepII

Plasmid

#196667

Purpose

Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV-cleavable di-strep tag II

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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