We narrowed to 18,398 results for: Mut

1. pEB1-mYPet

   Plasmid

   #103988

   Purpose

   Plasmid encoding mYPet

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mYPet
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F46L, I47L, …

   Promoter

   proC

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCHW110033

   Plasmid

   #222441

   Purpose

   Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p8.9 NdeltaSB D185K/D186L

   Plasmid

   #132931

   Purpose

   p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

   Depositor

   Jeremy Luban

   Article

   De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

   Insert

   gag and pol D185K/D186L
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D185K/D186L

   Promoter

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-CaMKIIa-ChETA-EYFP

   Plasmid

   #26967

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T

   Promoter

   CaMKIIa

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDIV297

   Plasmid

   #204820

   Purpose

   AMA1 plasmid with amdS selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Insert

   amdS
   

   Use

   Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations (nt177T>C, nt879C>T)

   Promoter

   Aspergillus nidulans trpC promoter

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HsTAB182_K

   Plasmid

   #146777

   Purpose

   Mammalian Expression of HsTAB182

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsTAB182 (TNKS1BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 silent mutations compared to the sequence given…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJUMP27ts-1A(sfGFP)

   Plasmid

   #126975

   Purpose

   Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   RepA A56V thermosensitive mutation

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGK401red-­rtetTAK8N, L131L

    Plasmid

    #165968

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA HA FAK Y397F

    Plasmid

    #50503

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y397F

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Promoter

    Available Since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. MAC_N_STA5B

    Plasmid

    #167800

    Purpose

    Expresses STA5B in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    STA5B (STAT5B Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. V5-TurboID-Mfn1

    Plasmid

    #195155

    Purpose

    mammalian expression of TurboID tagged Mfn1

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    TurboID
    

    Use

    Tags

    Mfn1

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    CMV

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50471

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50467

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV2 and AAV8

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pReporter_12

    Plasmid

    #60572

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(-) FLIPQTV3.0 2m

    Plasmid

    #63731

    Purpose

    FLIPQTV3.0 glutamine sensor with R75MW220A mutations

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 2mM affinity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV:eGFP-CaM-uTEV1Δ(220-242)

    Plasmid

    #135461

    Purpose

    Mammalian expression of uFLARE protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-CaM-uTEV1Δ
    

    Use

    Tags

    eGFP, V5

    Expression

    Mammalian

    Mutation

    S219V mutation improves stability and S153N impro…

    Promoter

    CMV

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge